2 replies to this topic

[intern]

I am new to primer design.  I used OligoCalc to check my primers for self-complementarity and one of the sections gave me the thermodynamics of the primer.  Does anyone know what the parameters/ranges are for the deltaG, deltaH, and deltaS?

[susanna]

you can also use oligo to check the formation of primerdimers and primerhairpins: herefore in the case of primerdimer, the deltaG has to be higher than -5 kcal/mol for 3'end and higher dan -6 kcal/mol for internal. For hairpins, the deltaG must be higher than -2 kcal/mol for 3'en en higher dan -3 kcal/mol for internal hairpins.

[intern]

Thank you