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mammalian promoter


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#1 cupidstunt

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Posted 13 November 2009 - 02:32 AM

Hi all,

I have a gene in PCR4-TOPO vector. It has got T3 and T7 promoter. Are these mammalian promoters? I wanted to transfect but am not sure if this is an expression vector. The manual dosent say anything about it. Also it has a LacZ operon. Whats the use of it?

pls pardon my naivity.

Thanks

#2 phage434

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Posted 13 November 2009 - 04:52 AM

No, these are not mammalian promoters, and it will not express in mammalian cells. LacZ is useful to determine the presence of the insert on initial cloning.




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