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Cloning and Expression Method Survey


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#1 Adrian K

Adrian K

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Posted 12 November 2009 - 06:28 PM

Hi all,

I am trying to ask your opinions as I was trying to compare which is the best Expression kits/vector that you are using and if you would like to suggest and introduce others to use it, also your opinion about the PROS and CONS of the kits.

Thank you.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#2 Adrian K

Adrian K

    Legendary Graduate Beggar

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 708 posts
28
Excellent

Posted 12 November 2009 - 06:31 PM

Ok, I come first:

IMHO my expression study, I use pBAD TOPO TA expression kit. It is good for me but sometimes the competent cells doesn't really express my cloned protein. It is easy to perform but is quite expensive.

Will I recommend to others: perhaps.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434




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