I'm due to start working with Haemophilus influenze soon so I've done some preliminary plating on chocolate agar as I believe this is the main medium to grow it on. However, the colonies appear VERY small, transparent and are extremely difficult to see even through the lid of the petri dish with a light box underneath. Does anyone have any suggestions on making the colonies easier to see for my upcoming colony counts that I will have to be doing? All help is very welcome!
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Colony counting with Haemophilus influenzae
Started by kingkeiffy, Nov 12 2009 01:37 PM
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Posted 13 November 2009 - 05:54 AM
kingkeiffy, on Nov 12 2009, 01:37 PM, said:
I'm due to start working with Haemophilus influenze soon so I've done some preliminary plating on chocolate agar as I believe this is the main medium to grow it on. However, the colonies appear VERY small, transparent and are extremely difficult to see even through the lid of the petri dish with a light box underneath. Does anyone have any suggestions on making the colonies easier to see for my upcoming colony counts that I will have to be doing? All help is very welcome!
Maybe you can try to cultivate them on Blood agar it works fine for SP or add some ISovitalex in your chocolate agar that's what we do in our lab
http://findarticles.com/p/articles/mi_qa38...9/ai_n32063249/
