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free software for analyzing FACS files


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13 replies to this topic

#1 yobou

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Posted 12 November 2009 - 11:10 AM

Dear All
I would like to know if there is a free software (equivalent to CellQuest) for performing cell cycle analysis of FACS files aquired by FACScalibur
thanks

Edited by yobou, 12 November 2009 - 01:36 PM.


#2 elpollodiablo

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Posted 13 November 2009 - 05:32 AM

Dear All
I would like to know if there is a free software (equivalent to CellQuest) for performing cell cycle analysis of FACS files aquired by FACScalibur
thanks


Try Weasel, a free, java-based program to analyse FACS data. I've used it, it's not as good as the commercial programs but it is free and has great functionality once you get used to it!

#3 canotto

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Posted 13 November 2009 - 06:36 AM

Dear All
I would like to know if there is a free software (equivalent to CellQuest) for performing cell cycle analysis of FACS files aquired by FACScalibur
thanks


Try Weasel, a free, java-based program to analyse FACS data. I've used it, it's not as good as the commercial programs but it is free and has great functionality once you get used to it!


are you sure that Weasel is free???

#4 elpollodiablo

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Posted 18 November 2009 - 04:45 AM

Dear All
I would like to know if there is a free software (equivalent to CellQuest) for performing cell cycle analysis of FACS files aquired by FACScalibur
thanks


Try Weasel, a free, java-based program to analyse FACS data. I've used it, it's not as good as the commercial programs but it is free and has great functionality once you get used to it!


are you sure that Weasel is free???


Sorry you're right. It's shareware, but there is no timer on it, so there is no technical problem with using it indefinitely, only a moral problem.

#5 blotted

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Posted 22 December 2009 - 05:03 AM

Cytomation Summit I think is free!

#6 Doki

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Posted 22 December 2009 - 05:17 AM

Hi Blotted, Where can we download that from? Googled it but could not find anywhere to download from though it sounds like free.
Simple living, highnot thinking

#7 blotted

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Posted 22 December 2009 - 05:28 AM

Hi Blotted, Where can we download that from? Googled it but could not find anywhere to download from though it sounds like free.


The software is from Dako, but I don't know if you can find here, because I get from the flow cytometry facility core of my university. Try in the website:

http://www.dako.com/

Normally I think that they distribute the software with cytometers, so maybe you should ask to your facility core responsible.

#8 Doki

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Posted 22 December 2009 - 05:33 AM

I looked around in that site already. Didn't have a clue. Will try again.

How good is it?

Better than WinMDI? I am looking for a free WinMDI alternative. None yet!
Simple living, highnot thinking

#9 Perttu

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Posted 31 March 2010 - 11:45 PM

Somehow the development of free flow cytometry analysis softwares have been very quiet (compared to e.g. microscopy: for instance ImageJ and BioimageXD, both open source projects).

Now I'm trying to do my part to change this. Check Flowing Software:

http://www.flowingsoftware.com

It's the project for developing new, innovative tool for flow cytometry data analysis. All basic tools are available, but you can go much further; the main goal is to develop easy ways for effective, but easy high throughput analysis.

Yes, it's free! :rolleyes:

#10 Doki

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Posted 01 April 2010 - 01:51 AM

Perttu, Thank U for this. I will try it out and give U a feedback soon.
Simple living, highnot thinking

#11 bozava

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Posted 21 April 2011 - 12:45 AM

Thanks Perttu, I am now trying flowing. It is quite confusing at first, but the tutorial video found on their website is really helpful. The software looks fairly robust, with all the functions I need. I would recommend it.

#12 mouse murderer

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Posted 27 April 2011 - 07:13 AM

I downloaded FlowingSoftware and watched the online orientation video and this software is definitely easy to use and full of all the capabilities I need.

#13 Adk

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Posted 15 November 2011 - 05:27 AM

Hi
I just downloaded the Flowing Software. Now I am trying to work on my data - I create a histogram (or a dot plot) and then I am opening my FCS file and then nothing appears in my histogram/dot plot - any suggestions?
Thanks

#14 acquire

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Posted 04 December 2011 - 10:32 PM

mine also same problem .nothing appears..like Adk..suggest us.
iam using LMD files from FC500.which software i should download for LMD files?




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