13 replies to this topic

[yobou]

Dear All
I would like to know if there is a free software (equivalent to CellQuest) for performing cell cycle analysis of FACS files aquired by FACScalibur
thanks

Edited by yobou, 12 November 2009 - 01:36 PM.

[elpollodiablo]

yobou, on Nov 12 2009, 11:10 AM, said:

Dear All
I would like to know if there is a free software (equivalent to CellQuest) for performing cell cycle analysis of FACS files aquired by FACScalibur
thanks

Try Weasel, a free, java-based program to analyse FACS data. I've used it, it's not as good as the commercial programs but it is free and has great functionality once you get used to it!

[canotto]

elpollodiablo, on Nov 13 2009, 02:32 PM, said:

yobou, on Nov 12 2009, 11:10 AM, said:

Dear All
I would like to know if there is a free software (equivalent to CellQuest) for performing cell cycle analysis of FACS files aquired by FACScalibur
thanks

Try Weasel, a free, java-based program to analyse FACS data. I've used it, it's not as good as the commercial programs but it is free and has great functionality once you get used to it!

are you sure that Weasel is free???

[elpollodiablo]

canotto, on Nov 13 2009, 06:36 AM, said:

elpollodiablo, on Nov 13 2009, 02:32 PM, said:

yobou, on Nov 12 2009, 11:10 AM, said:

Dear All
I would like to know if there is a free software (equivalent to CellQuest) for performing cell cycle analysis of FACS files aquired by FACScalibur
thanks

Try Weasel, a free, java-based program to analyse FACS data. I've used it, it's not as good as the commercial programs but it is free and has great functionality once you get used to it!

are you sure that Weasel is free???

Sorry you're right. It's shareware, but there is no timer on it, so there is no technical problem with using it indefinitely, only a moral problem.

[blotted]

Cytomation Summit I think is free!

[Doki]

Hi Blotted, Where can we download that from? Googled it but could not find anywhere to download from though it sounds like free.

[blotted]

Nabi, on Dec 22 2009, 02:17 PM, said:

Hi Blotted, Where can we download that from? Googled it but could not find anywhere to download from though it sounds like free.

The software is from Dako, but I don't know if you can find here, because I get from the flow cytometry facility core of my university. Try in the website:

http://www.dako.com/

Normally I think that they distribute the software with cytometers, so maybe you should ask to your facility core responsible.

[Doki]

I looked around in that site already. Didn't have a clue. Will try again.

How good is it?

Better than WinMDI? I am looking for a free WinMDI alternative. None yet!

[Perttu]

Somehow the development of free flow cytometry analysis softwares have been very quiet (compared to e.g. microscopy: for instance ImageJ and BioimageXD, both open source projects).

Now I'm trying to do my part to change this. Check Flowing Software:

http://www.flowingsoftware.com

It's the project for developing new, innovative tool for flow cytometry data analysis. All basic tools are available, but you can go much further; the main goal is to develop easy ways for effective, but easy high throughput analysis.

Yes, it's free!

[Doki]

Perttu, Thank U for this. I will try it out and give U a feedback soon.

[bozava]

Thanks Perttu, I am now trying flowing. It is quite confusing at first, but the tutorial video found on their website is really helpful. The software looks fairly robust, with all the functions I need. I would recommend it.

[mouse murderer]

I downloaded FlowingSoftware and watched the online orientation video and this software is definitely easy to use and full of all the capabilities I need.

[Adk]

Hi
I just downloaded the Flowing Software. Now I am trying to work on my data - I create a histogram (or a dot plot) and then I am opening my FCS file and then nothing appears in my histogram/dot plot - any suggestions?
Thanks

[acquire]

mine also same problem .nothing appears..like Adk..suggest us.
iam using LMD files from FC500.which software i should download for LMD files?