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Bacteroides mutant construction


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#1 dreamerbac

dreamerbac

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Posted 12 November 2009 - 10:27 AM

Hi
I'm wondering if I want to make a mutant in Bacteroides uisng SOE PCR. Would 800-1000bp fragment on each flanking side be enough for an efficient homologous recombination, like E.coli? Or it has to be around 2000-3000bp?
Thanks!!




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