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Started by ulujm, Nov 12 2009 09:40 AM

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#1 ulujm

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Posted 12 November 2009 - 09:40 AM

Hi in non reducing condition my actin signal is low (alpha actine)

any idea why? is because it's in non reducing condition?

beta actin is it better?

#2 bob1

    Hmmm, I think it's working

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Posted 12 November 2009 - 03:06 PM

Possibly, it would depend on your antibody as to whether the epitope is exposed under non-reducing conditions.
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