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What retrovirus vetor can have 7.5kb insert


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#1 Gongfu Panda

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Posted 11 November 2009 - 03:01 PM

I am working on a large protein whose cDNA is 7.5kb big. I need subclone the cDNA into one retrovirus vector. Can anyone give some suggestions about retrovirus vectors that I can use?
Thanks in advance.

#2 elpollodiablo

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Posted 12 November 2009 - 03:51 AM

A standard lentiviral backbone will allow ~8kb of insert.




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