10 replies to this topic

[Tamarind]

Help...........
I am trying to make a 10mls of ATP 10Mm solution the MW is 605.2

My balance is in grams and I am struggling...
any help would be good.
  

[Tamarind]

Having problems making a stable 10ml 10Mm solution.
MW is 605.2 my scales are in grams
Help.....

[Gerard]

10 mmol/l means 6.05g/l
balance in grams then 10 ml is impossible

[phage434]

10 mM (note the case, not Mm) is 1/100 mole per liter, so you would need 605/100 = 6.05 grams/liter to make a 10 mM solution.  You need 10 ml, or 1/100 of a liter, so  you need .0605 g or 60.5 mg of ATP in 10 ml for your solution.  I would not make it this way, but would instead make a more concentrated solution and dilute it.  For example, you could make a 1 M solution with 0.605 grams in 1 ml.  Then you could make the 10 mM solution by diluting 100x.  Since you want 10 ml, you put 100 ul of 1 M ATP soltution in 9.9 ml of water.  You need to think about the stability of your high concentration stock solution -- in this case, freezing at -20 will be stable for a few months, but you would want to avoid too many freeze/thaw cycles.

[Tamarind]

Gerard, on Nov 11 2009, 06:54 AM, said:

10 mmol/l means 6.05g/l
balance in grams then 10 ml is impossible

So I need to make  litre?

[Tamarind]

Can't i take it down to 0.06052g to get 10mls?

[Tamarind]

phage434, on Nov 11 2009, 07:30 AM, said:

10 mM (note the case, not Mm) is 1/100 mole per liter, so you would need 605/100 = 6.05 grams/liter to make a 10 mM solution.  You need 10 ml, or 1/100 of a liter, so  you need .0605 g or 60.5 mg of ATP in 10 ml for your solution.  I would not make it this way, but would instead make a more concentrated solution and dilute it.  For example, you could make a 1 M solution with 0.605 grams in 1 ml.  Then you could make the 10 mM solution by diluting 100x.  Since you want 10 ml, you put 100 ul of 1 M ATP soltution in 9.9 ml of water.  You need to think about the stability of your high concentration stock solution -- in this case, freezing at -20 will be stable for a few months, but you would want to avoid too many freeze/thaw cycles.

Thank you sooooooooooooo much, I will try this in a moment. Sorry about the case, was gettin desperate.

[Tamarind]

I tried the 0.605g in 1ml.
ATP does not dissolve properly.
I did dilute down from there and have frozen in 2ml aliquots. will see what happens when I try the samples.

I resorted to the other method using 0.0605g in 10mls ,
then aliquot into 2mls and freeze.
Again will see what I get.

Thanks for the help

[mdfenko]

Tamarind, on Nov 11 2009, 11:40 AM, said:

I tried the 0.605g in 1ml.
ATP does not dissolve properly.
I did dilute down from there and have frozen in 2ml aliquots. will see what happens when I try the samples.

I resorted to the other method using 0.0605g in 10mls ,
then aliquot into 2mls and freeze.
Again will see what I get.

Thanks for the help

you have to adjust the pH to ~7 or higher to get it all in solution at 1M (we adjust to 7).

[arc1]

mdfenko, on Nov 13 2009, 10:20 PM, said:

Tamarind, on Nov 11 2009, 11:40 AM, said:

I tried the 0.605g in 1ml.
ATP does not dissolve properly.
I did dilute down from there and have frozen in 2ml aliquots. will see what happens when I try the samples.

I resorted to the other method using 0.0605g in 10mls ,
then aliquot into 2mls and freeze.
Again will see what I get.

Thanks for the help

you have to adjust the pH to ~7 or higher to get it all in solution at 1M (we adjust to 7).

[arc1]

Hi
Well we've seen that ATP stocks are stable in acidified water..pH~3 !!
and there is no problem of solubility also
All the best