1 reply to this topic

[melody89]

I feel kind of ridiculous posting this, but when I try pipetting Sepharose beads after washing and spinning them...well, I can't.  They don't pipette up.  I've cut the tips.  Do I leave some of the wash buffer in there or what?  I'm just using these beads initially to cut down on nonspecific binding.  I don't have any problems when I need to pipette PGS beads.  Suggestions?  (Sorry if this seems pretty elementary..)

[Prep!]

well i ve not done tat but may be a paste will make it more difficult to pipette. So better leave some wash buffer as little as can be pipetted out!!! Alternatively try taking it out by a pastuer pipette if the volume does not need to be accurate!!