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[Gu_Margaret]

A great exon intron boundary search engine to share with everyone!

Q: Some genes are on the negative strand of the genomic sequence.
How can we get the sequence on the negative strand and have the sequence cutted according the exon/intron boundary and have exon/intron sorted from 5' to 3', without paying an arm and a leg?



A: http://www.mybioinfo...arch_symbol.php
This website will let you have everything in a tabular format and links to the sequences with exon/intron boundary defined.

You get + strand sequence if the gene is on + strand.
You get - strand sequence if the gene is on - strand.

Exon/intron are sorted from 5' to 3', whether or not the gene is on + or - strand.
Even UTR and CDS are shown clearly.