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Recycling coomassie blue


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5 replies to this topic

#1 selaphiel

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Posted 09 November 2009 - 01:39 PM

Hi everyone,

I have a simple question, When you stain your SDSpage gel with coomassie blue, do you recycle the solution?
I know we recycle ponceau solution but I have some doubts about the coomassie blue.

thanks :P

#2 Gerard

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Posted 09 November 2009 - 01:59 PM

You can use it several times if you wish, at our protein-HRE we used it until there was to much precipitation on the gel
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

#3 mdfenko

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Posted 10 November 2009 - 07:16 AM

we used to reuse coomassie (r250) but found that our results would vary so we started using it once and discard.

Edited by mdfenko, 10 November 2009 - 07:17 AM.

talent does what it can
genius does what it must
i do what i get paid to do

#4 Helios

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Posted 10 November 2009 - 11:19 PM

we reuse coomassie regularly in our lab....havn't seen any problems till now

#5 Prep!

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Posted 11 November 2009 - 12:57 AM

depends on the purpose... universally it is reyused.. but it is advisable for a identity method... for a method to be used as purity, if the solution is recycled more number of times (no of times here has to be researched) the LOD and LOQ can be compromised!!!
Best luck!!
Support bacteria - They are the only culture some people have!!!
Cheers!!!

#6 selaphiel

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Posted 13 November 2009 - 07:51 AM

Thanks, that's what I thought but I wanted confirmation.
I'm going to reuse it.

Have a nice weekend everybody




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