Does anyone have any experience in choosing qRT-PCR housekeeping genes for neuronal cultures? We already have tried b-actin and GAPDH but are not happy with them so thinking of trying some others... any info would be of great help!
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Posted 09 November 2009 - 12:07 PM
scistudent, on Nov 9 2009, 01:24 PM, said:
Does anyone have any experience in choosing qRT-PCR housekeeping genes for neuronal cultures? We already have tried b-actin and GAPDH but are not happy with them so thinking of trying some others... any info would be of great help!
An isoform of beta-tubulin may work well, 1 or 3 especially.
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