I plan to prepare conditioned media from tumor cells for later use in culture on stroma cells to perform different assays. I was told to add protease inhibitor to the conditioned media before freezing it at -80 C. That surprises me because I would add protease inhibitor if I wanted to extract proteins but not when using it again in culture, I think proteases inhibitor could interfere with the effect the conditioned media will have or not when I will use it in culture. Anybody has an idea if I shoud effectively add protease inhibitor or not?
thanks a lot for your help
Edited by sabi67, 07 November 2009 - 03:51 PM.