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Confusing bands from PCR


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#31 lab rat

lab rat

    Why does a science forum not have pictures of mice and rats?

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Posted 11 December 2009 - 01:04 PM

Hi tomato,

This seems off-the-wall, but one of the research assistants in our plant science department asked how old your primers were. She had a few sets that would stop working after a month or two--even if she used them only once and left them frozen for a month before the second use--so she began aliquotting them and pulling out only what she needed. She had no explanation for the dropoff in efficacy; she just figured they were less stable than her other sets.

regards,

lab rat
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.




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