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LOH


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#1 SF_HK

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Posted 06 November 2009 - 01:19 AM

Hi,

Could somone please help me understand LOH. I understand thatone of the allele is either muated or deleted. So, if only one allele is mutated, does that imply reduced expression as opposed to complete deletion due to homozygous deletion. I'm a bit confused here. My gene shows reduced expression in cancer cell lines compared to normal cells and furthermore, the location of this gene of interest is on a chromosome that frequently experioences LOH in the cancer i'm studying. Basicaly what I'd like to know is can LOH also explain for reduced expression of gene analysis no doe sit explain for no expression?

Sorry if my question sounds silly.

#2 warsel

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Posted 09 November 2009 - 04:55 AM

Hi,

Could somone please help me understand LOH. I understand thatone of the allele is either muated or deleted. So, if only one allele is mutated, does that imply reduced expression as opposed to complete deletion due to homozygous deletion. I'm a bit confused here. My gene shows reduced expression in cancer cell lines compared to normal cells and furthermore, the location of this gene of interest is on a chromosome that frequently experioences LOH in the cancer i'm studying. Basicaly what I'd like to know is can LOH also explain for reduced expression of gene analysis no doe sit explain for no expression?

Sorry if my question sounds silly.


I am not an expert on this particularly, but my understanding is that by mutation of one allele (not necessarily causing any difference in expression but a difference in activity) and deletion of the other allele you get the LOH where the cancer cell is now got rid of the gene(function) in question - most likely in the case of a tumor supressor.




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