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Chromosomes in metaphase


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#1 ForEpi

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Posted 05 November 2009 - 07:42 AM

What happens to DNA methylation when cells are in metaphase?

The reason I'm asking this is because I want to find out whether we can use FACS to isolate chromosomes and for next generation sequencing without having to sequence the whole genome...

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#2 methylnick

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Posted 24 November 2009 - 01:03 PM

that is a good question and I don't think anyone knows.

one would assume by metaphase the copying of epigenetic information post S-phase is complete, so nothing happens to it at metaphase.

#3 ForEpi

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Posted 24 November 2009 - 11:07 PM

Thanks for your reply Nick. I've decided to go for another technique: sequence capture arrays. Novel and exciting :-)
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