I was trying to validated a custom designed taqman probe -- I ran a SYBR green melting curve and independently ran a RT-PCR reaction using the same RNA then ran the product on a gel. The interesting thing is, I got only 1 peak in the SYBR melting curve, which usually means that there's only 1 PCR product, but I got 3 distinct bands on my gel! Other than my <100 bp expected PCR product, I also saw a 200 bp and a 600 bp band. Any idea what's going on here?
Edited by konnicska, 04 November 2009 - 02:20 PM.