4 replies to this topic

[Mushin]

My original choice was abcam's ab8580, but this is out of stock and their staff say they have no idea when new batches will be available. Upstate/Millipore's 07-473 is also now out of stock, and I'm waiting on their customer services to let me know if more will be available.

These two antibodies are by far the most popular I've seen in ChIP experiments. I will be using ChIP DNA for next gen sequencing so it's absolutely critical the antibody works well. Does anyone have a suggestion for an alternative, that has been used in a high profile publication, or you have personal experience with?

Thanks.

[epi123]

Mushin, on Nov 4 2009, 07:54 AM, said:

My original choice was abcam's ab8580, but this is out of stock and their staff say they have no idea when new batches will be available. Upstate/Millipore's 07-473 is also now out of stock, and I'm waiting on their customer services to let me know if more will be available.

These two antibodies are by far the most popular I've seen in ChIP experiments. I will be using ChIP DNA for next gen sequencing so it's absolutely critical the antibody works well. Does anyone have a suggestion for an alternative, that has been used in a high profile publication, or you have personal experience with?

Thanks.

Invitrogen and Cell signalling have this antibody as well but don't know any publications.

[annabellak]

I've used the cell signaling antibody for ChIP and it worked really well - better than upstate anyway!

[epi123]

other source for H3-K4Me3 antibody- Invitrogen, Epigentek

[jamessmith01]

Just to update, Upstate/Millipore's 07-473 is now in stock.