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Suitable antibody for H3K4me3?


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4 replies to this topic

#1 Mushin

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Posted 04 November 2009 - 07:54 AM

My original choice was abcam's ab8580, but this is out of stock and their staff say they have no idea when new batches will be available. Upstate/Millipore's 07-473 is also now out of stock, and I'm waiting on their customer services to let me know if more will be available.

These two antibodies are by far the most popular I've seen in ChIP experiments. I will be using ChIP DNA for next gen sequencing so it's absolutely critical the antibody works well. Does anyone have a suggestion for an alternative, that has been used in a high profile publication, or you have personal experience with?

Thanks.

#2 epi123

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Posted 07 November 2009 - 10:20 AM

My original choice was abcam's ab8580, but this is out of stock and their staff say they have no idea when new batches will be available. Upstate/Millipore's 07-473 is also now out of stock, and I'm waiting on their customer services to let me know if more will be available.

These two antibodies are by far the most popular I've seen in ChIP experiments. I will be using ChIP DNA for next gen sequencing so it's absolutely critical the antibody works well. Does anyone have a suggestion for an alternative, that has been used in a high profile publication, or you have personal experience with?

Thanks.



Invitrogen and Cell signalling have this antibody as well but don't know any publications.

#3 annabellak

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Posted 08 March 2010 - 03:04 PM

I've used the cell signaling antibody for ChIP and it worked really well - better than upstate anyway!

#4 epi123

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Posted 10 March 2010 - 11:16 AM

other source for H3-K4Me3 antibody- Invitrogen, Epigentek

#5 jamessmith01

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Posted 11 March 2010 - 09:16 AM

Just to update, Upstate/Millipore's 07-473 is now in stock.




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