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Primary cerebellar neurons and proteasome assay


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#1 katenkak

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Posted 04 November 2009 - 03:58 AM

Hi all!
I have to perform cell-based proteasome Glo assay on primary cerebellar granular neruons. They grow at their best on poly-D-lysine, which in low amount can by itself stimulate proteasome activity. Does any of you know what else I can successfully grow my neurons on in order to make the assay? Many thanks for advice!

#2 gfischer

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Posted 04 November 2009 - 06:39 AM

I use Poly-L-Lysine for sensory neuron culture, which should work well for cerebellar neurons as well. I've also seen neurons cultured with laminin, poly-ornithine, or collagen for attachment, though I don't know whether any of these will stimulate proteasomes.
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#3 katenkak

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Posted 04 November 2009 - 10:53 AM

Thanks for reply. I have another question: why people mostly use namely poly-lysine (L or D) and not other substrates? Do you know or have a reference with explanation?
Only poly-lysine would stimulate proteasome activity.

I use Poly-L-Lysine for sensory neuron culture, which should work well for cerebellar neurons as well. I've also seen neurons cultured with laminin, poly-ornithine, or collagen for attachment, though I don't know whether any of these will stimulate proteasomes.






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