Primary cerebellar neurons and proteasome assay
Posted 04 November 2009 - 03:58 AM
I have to perform cell-based proteasome Glo assay on primary cerebellar granular neruons. They grow at their best on poly-D-lysine, which in low amount can by itself stimulate proteasome activity. Does any of you know what else I can successfully grow my neurons on in order to make the assay? Many thanks for advice!
Posted 04 November 2009 - 06:39 AM
then heaven will be yours, before you meet your end
Posted 04 November 2009 - 10:53 AM
Only poly-lysine would stimulate proteasome activity.
I use Poly-L-Lysine for sensory neuron culture, which should work well for cerebellar neurons as well. I've also seen neurons cultured with laminin, poly-ornithine, or collagen for attachment, though I don't know whether any of these will stimulate proteasomes.