Hi all.. i ve got a crazy (may be even stupid) doubt all of a sudden (this happens when u analyse thousaunds of data )

I desire a certain amount A (i have added this component suppose its units is mg/ml)

I get the value from assay as B (from the graph equation again in mg/ml)

If i do B/A, the percentage of it is Accuracy... and 100-the answer is the inaccuracy!!!

If I do A/B, what does the answer signify???

Thanx.. hope i dint cinfuse!!!

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# Accuracy???!!!

Started by Prep!, Nov 03 2009 09:21 PM

4 replies to this topic

### #1

Posted 03 November 2009 - 09:21 PM

**Support bacteria - They are the only culture some people have!!!**

**Cheers!!!**

### #2

Posted 03 November 2009 - 10:43 PM

suppose i did an ELISA

my standard was 2.5,5,10,25,50 ng/ml.

I plot a graph of the resopnse

i back calculate from the graph equation (linear or 4PL)

for 5 ng/ml standard, i get a value of 4 ng/ml

so the accuracy by which i could detect the standard (in other terms %recovery) is 4/5*100=80%. So the inacuracy is 20%

Now the inverse that is 5/4*100 is 125%. What does this value signify!!!

I hope its clear now!!!

my standard was 2.5,5,10,25,50 ng/ml.

I plot a graph of the resopnse

i back calculate from the graph equation (linear or 4PL)

for 5 ng/ml standard, i get a value of 4 ng/ml

so the accuracy by which i could detect the standard (in other terms %recovery) is 4/5*100=80%. So the inacuracy is 20%

Now the inverse that is 5/4*100 is 125%. What does this value signify!!!

I hope its clear now!!!

**Support bacteria - They are the only culture some people have!!!**

**Cheers!!!**

### #3

Posted 04 November 2009 - 01:49 AM

To further simplify

value obtained/value desired*100. This gives acuracy

I wanted to ask what will value desired/value obtained give!!!

Both give different results... So the confusion!!!

value obtained/value desired*100. This gives acuracy

I wanted to ask what will value desired/value obtained give!!!

Both give different results... So the confusion!!!

**Support bacteria - They are the only culture some people have!!!**

**Cheers!!!**

### #4

Posted 05 November 2009 - 09:12 PM

To further simplify

value obtained/value desired*100. This gives acuracy

I wanted to ask what will value desired/value obtained give!!!

Both give different results... So the confusion!!!

..hi..

warning:not an expert just putting across wht i feel about this:

does'nt it signifies "bias" in the data,???

### #5

Posted 05 November 2009 - 09:15 PM

hi rick... long time

ya it actually describes the error % in the data.. so was just wondering if we can relate it to accuracy!!!

if i have no spike control.. and suppose its only the standard... wat term wud we give this value?? accuracy% (over or under-watever)?? or error% - bias in your terms!!!

ya it actually describes the error % in the data.. so was just wondering if we can relate it to accuracy!!!

if i have no spike control.. and suppose its only the standard... wat term wud we give this value?? accuracy% (over or under-watever)?? or error% - bias in your terms!!!

**Support bacteria - They are the only culture some people have!!!**

**Cheers!!!**