Edited by Andrew, 03 November 2009 - 08:40 PM.
T cell transduction
Posted 03 November 2009 - 08:39 PM
Posted 04 November 2009 - 01:01 AM
I want to transduce human Tcells with a Foxp3 shRNA. I've heard that Tcells are very hard to transduce, does anyone have any tips?
I'd guess you could do it with a HIV-based lentiviral vector system, as it is already adapted to transducing non-dividing T-cells.
Posted 04 November 2009 - 06:41 PM
With activated T cells, I have gotten up to 70% transduction efficiencies with retrovirus infections. 60 minutes spinfection with 1 million cells and 1 mL viral sup. 8 ug/mL of polybrene.
Posted 05 November 2009 - 06:17 PM
Edited by Functional Screens, 05 November 2009 - 06:17 PM.