3 replies to this topic

[mapleleaf4]

Hi all,
I've been using Amersham Full-Range Rainbow Molecular Weight marker as my ladder in my gels. However, this doesn't show up too well when stained with Coomassie Blue. Do you have any recommendations as to other types of ladders that will show up brightly? Thanks.

[Prep!]

I normally use Bio-Rad prestained and ubnstained broad range markers (ready to use)
They are very reproducible and reliable

[dllinpat]

mapleleaf4, on Nov 4 2009, 05:44 AM, said:

Hi all,
I've been using Amersham Full-Range Rainbow Molecular Weight marker as my ladder in my gels. However, this doesn't show up too well when stained with Coomassie Blue. Do you have any recommendations as to other types of ladders that will show up brightly? Thanks.

I've been using bio-rad "precision plus protein all blue standards" for a long time, it does work well. 2microlitter is good enough.

[Feelcontraire]

mapleleaf4, on Nov 3 2009, 12:44 PM, said:

Hi all,
I've been using Amersham Full-Range Rainbow Molecular Weight marker as my ladder in my gels. However, this doesn't show up too well when stained with Coomassie Blue. Do you have any recommendations as to other types of ladders that will show up brightly? Thanks.

There are ladder's ready to be coomasie stained and they are the best choice for accurate weight stimation.
I remember Sigma has some, probably every company has some.