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SDS ladder


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3 replies to this topic

#1 mapleleaf4

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Posted 03 November 2009 - 12:44 PM

Hi all,
I've been using Amersham Full-Range Rainbow Molecular Weight marker as my ladder in my gels. However, this doesn't show up too well when stained with Coomassie Blue. Do you have any recommendations as to other types of ladders that will show up brightly? Thanks.

#2 Prep!

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Posted 03 November 2009 - 09:08 PM

I normally use Bio-Rad prestained and ubnstained broad range markers (ready to use)
They are very reproducible and reliable
Support bacteria - They are the only culture some people have!!!
Cheers!!!

#3 dllinpat

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Posted 04 November 2009 - 12:47 AM

Hi all,
I've been using Amersham Full-Range Rainbow Molecular Weight marker as my ladder in my gels. However, this doesn't show up too well when stained with Coomassie Blue. Do you have any recommendations as to other types of ladders that will show up brightly? Thanks.

I've been using bio-rad "precision plus protein all blue standards" for a long time, it does work well. 2microlitter is good enough.

#4 Feelcontraire

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Posted 18 November 2009 - 06:10 PM

Hi all,
I've been using Amersham Full-Range Rainbow Molecular Weight marker as my ladder in my gels. However, this doesn't show up too well when stained with Coomassie Blue. Do you have any recommendations as to other types of ladders that will show up brightly? Thanks.


There are ladder's ready to be coomasie stained and they are the best choice for accurate weight stimation.
I remember Sigma has some, probably every company has some.




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