DAPI and Flow Cytometry
Posted 03 November 2009 - 09:16 AM
does any one have suggestion how to detect dapi with flow cytometry? should it be one plot (FL4) or two plot (SSC/ FL4)?
can flow cytometry detect the apoptotic bodies using dapi staining?
Posted 03 November 2009 - 11:34 PM
Posted 04 November 2009 - 12:34 AM
thanks but how do you analyse your cell population? when apoptotic bodies formed in a population, what should I see?
of course there is a rise in SSC but what about FL4?
Posted 05 November 2009 - 09:40 PM
Posted 22 December 2009 - 05:17 AM
you can see apoptotic cells quantifying subG1 peak. For this purpose I recomend you to use PI.