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I’m thinking about doing a non-gel based technique approach to identify cell wall proteins (most of them are basic and highly glycosilated). Because of these characteristics, proteins doesn’t go easily inside of isoelectric focusing gels, so we will lose a lot of such proteins in a 2D approach.
I’m thinking about using iTRAQ technology, but until this point I don’t know how high would be the cost for analyzing 3 control and 3 treated samples. Do someone of you know, if the cost of such non-gel based techniques are much higher than a usual DIGE experiment? I refer to the cost of chemicals.
Thank you for your support.
Edited by kern, 03 November 2009 - 01:41 AM.
