Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Isolation of genomic DNA from microbial consortia


  • Please log in to reply
2 replies to this topic

#1 vettri

vettri

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 02 November 2009 - 07:21 PM

Hi people i am doing my mini project in solid waste mangement.....
Is there any specific protocols to isolate the total DNA of the microbial consortia from the sample?
Karthik

#2 gebirgsziege

gebirgsziege

    I like fungi

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 392 posts
25
Excellent

Posted 02 November 2009 - 10:35 PM

There are as many protocols as working groups. I would suggest you to do a literatur research, as the sucess of the method used is highly dependent on the sample AND the microbes you want to isolate (i.e. bacteria, archea, fungi,.....) AND the succesive methods used (i.e. PCR cloning, DGGE, SSCP, 454 sequencing,....).

I think your boss should provide you a protocol as well??
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)

#3 ShannonJ

ShannonJ

    member

  • Active Members
  • Pip
  • 24 posts
0
Neutral

Posted 04 November 2009 - 10:35 AM

Most people do a DNA extraction from the soil/sediment matrix either using a kit (faster, generally increased purity/yields) or phenol-chloroform (way cheaper) and then follow with a community analysis method. You need to begin by deciding which analysis method you're planning to use and how much money you have to spend on it. I know this is vague but its true, your advisor should be giving you some of these pieces of information from the get-go.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.