So it was my first time doing a WB. Since I had to rush to an important thing, I did not have time to develop chromogen after 2ndry antibody. I was told that its OK to dry the membrane and later develop color, which I did. We then found out that nobody has ever done that and people dry either before they start the antibody washes or once its completely done, not in the middle. Is there anyway to salvage the situation? People told to try "activate" the membrane with methanol, wash it couple of times with PBS/TBS and then try developing color. But I wanted to ask the community if anybody has done that. By the way I'm using PVDF membrane.
Thanks in advance for your tips and assistance!
Edited by jangajarn, 02 November 2009 - 02:14 PM.