Is it true that by adding a few nucleotides to the restriction sites of some endonucleases you will increase its efficiency?
I think I heard that in a lecture, but my Pi have never heard of it...so, I am confused now....
0
3 replies to this topic
#1
-
- Members
-
- 4 posts
member
Posted 02 November 2009 - 08:03 AM
HI
Is it true that by adding a few nucleotides to the restriction sites of some endonucleases you will increase its efficiency?
I think I heard that in a lecture, but my Pi have never heard of it...so, I am confused now....
Is it true that by adding a few nucleotides to the restriction sites of some endonucleases you will increase its efficiency?
I think I heard that in a lecture, but my Pi have never heard of it...so, I am confused now....
#2
-
- Active Members
-
- 536 posts
Veteran
Posted 02 November 2009 - 04:38 PM
You add the extra bases to the end ofteh RE site ,to give hte enzyme something to hold onto. Check out the NEb website and see how many extra bases are required to improve your Re's efficiency.
Be nice to your bureaucrats: they control your budgets...
#4
-
- Active Members
-
- 2,715 posts
Am I me???!!!
Posted 03 November 2009 - 11:09 PM
swanny, on Nov 3 2009, 06:08 AM, said:
You add the extra bases to the end ofteh RE site ,to give hte enzyme something to hold onto. Check out the NEb website and see how many extra bases are required to improve your Re's efficiency.
Tat was informative.. although i don work with RE's, this came as an interesting fact.. thanx for the thread canar
Support bacteria - They are the only culture some people have!!!
Cheers!!!
Cheers!!!
