Hi am attempting to localize a small 7kDa DNA binding protein in E. coli
cells via fluorescent microscopy. ....GFP and his patches on c or n terminus kills function.. I am thinking of using either genetically encoded florescent amino acid
on the back of the protein or using a His-patch on the protein and then use anti-His fluorescent anitbodies to localize the protein in vivo
also under consideration was the use of tetra cysteine
Does any one have any suggestions or advice concerning the methods i am contemplating?
Edited by Afroscience, 02 November 2009 - 07:43 AM.