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ELISA results


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5 replies to this topic

#1 miss-pcr

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Posted 02 November 2009 - 01:38 AM

Hi..
please tell me why my ELISA plate directly gives yellow color after adding the substrate

#2 pesji

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Posted 02 November 2009 - 02:56 AM

Which substrate and which secondary antibody do yu use ? Peroxidase labeled or Phosphatase ?

#3 swanny

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Posted 02 November 2009 - 04:40 PM

You may not have washed the enzyme off the plate.
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#4 tonix37

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Posted 03 November 2009 - 12:43 AM

Could be a problem of substract.
When you prepare substracte, can you see background on substracte solution before add it in the wells??


Which substrate and which secondary antibody do yu use ? Peroxidase labeled or Phosphatase ?



#5 almost a doctor

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Posted 03 November 2009 - 01:47 AM

how are you washing your plates before adding the substrate? most detergents interfere with chromogenic substrates so you need to wash your plates with dH2O a couple of times at the end before developing your plates

#6 miss-pcr

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Posted 03 November 2009 - 02:05 AM

Thanks for your all replys,yes my problem is in my substrate and washing procedure




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