Hi,
Just wondering if anyone is familar with a small flouresent tag which may be used to track a protein using real-time microscopy techniques?
I am finishing my honours project and i have found that EGFP affects the localization of my protein of interest (its much larger). I used the FLAG tag in my localization expriments. However, this tag is not appropriate for live cell imaging (atleast i dont think so)... as it needs an anti-body for detection. I am just wanting to make some recommendations for future directions of the project... and web searches have been unsucessful.
Any hints or ideas would be great.
Cheers.
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2 replies to this topic
#2
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CellSpecific.com
Posted 02 November 2009 - 07:50 AM
Quick question. How did you find that fusing EGFP altered the protein's normal cell trafficking path? Perhaps a bit more information on the protein of interest would be of help here.
#3
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Enthusiast
Posted 02 December 2009 - 04:02 PM
it comes to my mind HaloTag from Promega
on academic settings, prof K. Johnsson at EPFL Lausanne is making breakthroughs with new tag technologies for proteins.
96well
---my blog on genetically-encoded methods----
on academic settings, prof K. Johnsson at EPFL Lausanne is making breakthroughs with new tag technologies for proteins.
96well
---my blog on genetically-encoded methods----
Nature magazine. Do you qualify for a free subscription?
