
EXtraction of DNA bands from acrylamide gel
#1
Posted 01 November 2009 - 02:23 PM
#2
Posted 02 November 2009 - 07:01 AM
I am trying to extract DNA bands from 4% acrylamide gel, but I had no success. Does anybody thinks that the age of acrylamide gel (my gel stock solution is about a year old) can affect the extraction of DNA bands from the gel.
#3
Posted 03 November 2009 - 05:04 AM
In my experience, the acrylamide (if stored at 4C) should be fine. We also never "extracted" DNA from the gels, just cut the bands and placed them into 10mM Tris for 24-48hrs in the fridge. Over that time most of the DNA escaped from the gel into the liquid...at least enough for PCR. Hope that helps.
so we donīt need to use the gel extraction kits?
Awesome cost saver....
#4
Posted 03 November 2009 - 07:29 AM
so we donīt need to use the gel extraction kits?
Awesome cost saver....
#5
Posted 05 November 2009 - 11:08 PM