Co-transfection of two plasmids
Posted 01 November 2009 - 11:15 AM
I am trying to co-transfect (transient trasnfection) two different plasmids in to HEK-293T cells. One plasmid contains miRNA precursor and the other, 3'UTR target. Can any one briefly provide the protocol? Particularly, the amount of plasmids and lipofectamine are critical I guess.
Thanks in advance.
Posted 01 November 2009 - 08:16 PM
wow...thanks...what makes you think I haven't already tried it...the manual describes a the protocol for 2 plasmids and siRNA transfection and not 2 plasmids.
Go here, type in lipofectamine, choose reagent variant, download product sheet, read.
anyone else with useful suggestions please?
Posted 02 November 2009 - 04:18 PM
Posted 11 November 2009 - 08:47 AM
The protocol doesn't differ in any way! You will need to titrate the appropriate amount of each plasmid to add, but the protocol is identical.
How would I titrate the appropriate amount of each plasmid....?? I´m not asking for a protocol, just need to know the way of thinking or experimental setup and goal....
thanks in advance
Posted 11 November 2009 - 04:42 PM