Sayeh, on Nov 1 2009, 04:38 AM, said:
I am not sure if it is the right forum to post my question.
I am wondering if it is OK to reconstitute drugs in water. The drug that I am going to use is water soluble, but I am wondering if the water would affect the cells. What concentration of water would be harmful to the cells?
It is fine to dissolve your drugs in water, just make sure its sterile dH2O,
PBS probably works best and is the right osmotic pressure for the cells, again make sure its sterile by autoclaving.
The amount of water/PBS you use should have no affect as I pressume when you further dissolve your drugs in growth media when you treat your cells, thus the water/PBS will be dissolved to such a small concentration the affects would be negligable.
Also just a point, if your drugs are water soluble you could just dissovle them directly with your growth medium, have you though of doing this?