I need to culture mouse endothelial cells in low glucose media. I've cultured cells as first in a normal media, and is going to subculture them in low glucose media. Would this affect the growth of the cells?? What are the consequences??
What Is the best way to culture cells in low glucose media?? Is it prefered to start culturing in low glucose, or is it OK to do it as I've started -with normal media then subculturing in low glucose media- ???
Edited by Isra, 31 October 2009 - 01:56 PM.