How can I dissolve it????
Posted 30 October 2009 - 12:26 PM
I treat my cells (A549) with the organic pollutant benzo[a]pyrene and from the very first time i had the following problem. I use DMSO in order to dissolve B[a]P powder and make the stock solution (100mM). But after a week or less, B[a]P starts to precipitate in the bottom of the vial and its impossible to dissolve it again using vortex! I tried to warm it while vortexing or use ethanol instead of DMSO, but none of them worked. It's a problem that bothers me a whole year...
Does anyone of you use this compound or have any idea about how I could dissolve it?
Thank you in advance for providing any answers!!
Posted 01 November 2009 - 03:30 PM
You probably won't be able to re-dissolve the precipitate, it won't be benzo-pyrene!
Edited by bob1, 01 November 2009 - 03:34 PM.