2 replies to this topic

[borriello.87]

Hi all,
I would like to know if isotype controls have to be used
at the same concentrations of specific antibodies.

Thanks in advance!

[miBunny]

Yes

[CellSpecific.com]

In practice, you should use them at the same concentration. However, keep in mind that the recommended concentrations is really antibody amount in excess. Thus, if you're slightly off with your calculation, you should still be okay.