Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

best method for DNA extraction


  • Please log in to reply
3 replies to this topic

#1 noor albayan

noor albayan

    member

  • Active Members
  • Pip
  • 16 posts
0
Neutral

Posted 30 October 2009 - 02:47 AM

Hi all

What is the best method for DNA extraction??

CTAB techniqe or using kits??


and why????

#2 warsel

warsel

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 77 posts
0
Neutral

Posted 30 October 2009 - 05:46 AM

you didn't give enough information to answer that question.

"best" is relative to the application you want to use it for.

#3 ShannonJ

ShannonJ

    member

  • Active Members
  • Pip
  • 24 posts
0
Neutral

Posted 02 November 2009 - 07:04 AM

Also, what is your source material? Cells, water, soil, blood? Is your sample size limited so every ng of DNA is ultra-precious or are you interested in more cost-effective methods?

#4 gogreen

gogreen

    Somewhere I belong

  • Active Members
  • PipPipPipPipPip
  • 90 posts
0
Neutral

Posted 03 November 2009 - 02:52 AM

Thats right guys, there are quite a few parameters that u need to check
1. The downstream application
2. The cost factor
3. Ease of use
4. availability factor and there can be too many other factors

@ noor - Unless you specify what your requirement and constraints are, nobody can answer such a vague question! and definitely there won't be a consensus on the best method as different people use different kits and eventhough they know there are better kits available, most of 'em would still go with their tried and tested good ol' protocol!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.