Hi all
What is the best method for DNA extraction??
CTAB techniqe or using kits??
and why????
0
3 replies to this topic
#2
warsel
-
- Active Members
-
- 77 posts
Enthusiast
0
Neutral
Neutral
Posted 30 October 2009 - 05:46 AM
you didn't give enough information to answer that question.
"best" is relative to the application you want to use it for.
"best" is relative to the application you want to use it for.
#3
ShannonJ
-
- Active Members
-
- 24 posts
member
0
Neutral
Neutral
Posted 02 November 2009 - 07:04 AM
Also, what is your source material? Cells, water, soil, blood? Is your sample size limited so every ng of DNA is ultra-precious or are you interested in more cost-effective methods?
#4
gogreen
-
- Active Members
-
- 90 posts
Somewhere I belong
0
Neutral
Neutral
Posted 03 November 2009 - 02:52 AM
Thats right guys, there are quite a few parameters that u need to check
1. The downstream application
2. The cost factor
3. Ease of use
4. availability factor and there can be too many other factors
@ noor - Unless you specify what your requirement and constraints are, nobody can answer such a vague question! and definitely there won't be a consensus on the best method as different people use different kits and eventhough they know there are better kits available, most of 'em would still go with their tried and tested good ol' protocol!
1. The downstream application
2. The cost factor
3. Ease of use
4. availability factor and there can be too many other factors
@ noor - Unless you specify what your requirement and constraints are, nobody can answer such a vague question! and definitely there won't be a consensus on the best method as different people use different kits and eventhough they know there are better kits available, most of 'em would still go with their tried and tested good ol' protocol!
