Hi all,
I need your help.
I need a universal 16s rRNA primers which is already made as commercials.
Do you know which company have this pre-made Universal 16s rRNA primers? (this is not custom made)
Thank you
0
Universal 16s rRNA primers needed
Started by lojinsky, Oct 30 2009 12:17 AM
3 replies to this topic
#2
-
- Active Members
-
- 812 posts
Why does a science forum not have pictures of mice and rats?
Posted 30 October 2009 - 07:03 AM
Why don't you just pull them out of a publication?
Here's a link. http://aem.asm.org/c.../AEM.02220-08v1
Regards,
lab rat
Here's a link. http://aem.asm.org/c.../AEM.02220-08v1
Regards,
lab rat
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia.org/wiki/Python_(programming_language)" target="_blank">http://en.wikipedia.org/wiki/Python_(programming_language)</a>, accessed 25June2009.
#3
-
- Moderator
-
- 1,430 posts
Veteran
#4
-
- Active Members
-
- 21 posts
Enthusiast
Posted 01 November 2009 - 02:05 AM
hello
try following primers known as 27F and 1492R, they amplify a long 1.5 kb almost all of 16S gene, they work perfectly. do tell if u need short segments
27f AGA GTT TGA TCC TGG CTC AG
1492 TAC GGT TAC CTT GTT ACG ACT T
try following primers known as 27F and 1492R, they amplify a long 1.5 kb almost all of 16S gene, they work perfectly. do tell if u need short segments
27f AGA GTT TGA TCC TGG CTC AG
1492 TAC GGT TAC CTT GTT ACG ACT T
