Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

mTOR


  • Please log in to reply
4 replies to this topic

#1 mTOR

mTOR

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 30 October 2009 - 12:08 AM

Hi,

I am a student just started western blotting.
Is there anyone known about the successful blotting conditions for large protein (mTOR).
Help me, itís urgent!!!

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,782 posts
132
Excellent

Posted 30 October 2009 - 06:38 AM

use 0.05% sds and 10-20% methanol in the buffer.

if you have no concern about smaller proteins then you can transfer for a longer period.

but, you will probably never achieve 100% transfer.
talent does what it can
genius does what it must
i do what i get paid to do

#3 mTOR

mTOR

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 01 November 2009 - 11:14 PM

use 0.05% sds and 10-20% methanol in the buffer.

if you have no concern about smaller proteins then you can transfer for a longer period.

but, you will probably never achieve 100% transfer.


Thank you very much for your quick response and for your kindness. Your Information is helpfull for me !!

I did not add in sds yet. I will try to use sds in the buffer.

By the way, I have another qusetion.

Can I apply your conditions for the semi-dry system ?

Would you answer to my question?

#4 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,782 posts
132
Excellent

Posted 03 November 2009 - 08:06 AM

Can I apply your conditions for the semi-dry system ?

yes.
talent does what it can
genius does what it must
i do what i get paid to do

#5 mTOR

mTOR

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 04 November 2009 - 12:25 AM

Thank you very much!!

I will try it.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.