Hi,
I am a student just started western blotting.
Is there anyone known about the successful blotting conditions for large protein (mTOR).
Help me, it’s urgent!!!
0
4 replies to this topic
#2
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an elder
Posted 30 October 2009 - 06:38 AM
use 0.05% sds and 10-20% methanol in the buffer.
if you have no concern about smaller proteins then you can transfer for a longer period.
but, you will probably never achieve 100% transfer.
if you have no concern about smaller proteins then you can transfer for a longer period.
but, you will probably never achieve 100% transfer.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
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member
Posted 01 November 2009 - 11:14 PM
mdfenko, on Oct 30 2009, 06:08 PM, said:
use 0.05% sds and 10-20% methanol in the buffer.
if you have no concern about smaller proteins then you can transfer for a longer period.
but, you will probably never achieve 100% transfer.
if you have no concern about smaller proteins then you can transfer for a longer period.
but, you will probably never achieve 100% transfer.
Thank you very much for your quick response and for your kindness. Your Information is helpfull for me !!
I did not add in sds yet. I will try to use sds in the buffer.
By the way, I have another qusetion.
Can I apply your conditions for the semi-dry system ?
Would you answer to my question?
#4
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an elder
Posted 03 November 2009 - 08:06 AM
mTOR, on Nov 2 2009, 03:14 AM, said:
Can I apply your conditions for the semi-dry system ?
yes.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
