4 replies to this topic

[mTOR]

Hi,

I am a student just started western blotting.
Is there anyone known about the successful blotting conditions for large protein (mTOR).
Help me, it’s urgent!!!

[mdfenko]

use 0.05% sds and 10-20% methanol in the buffer.

if you have no concern about smaller proteins then you can transfer for a longer period.

but, you will probably never achieve 100% transfer.

[mTOR]

mdfenko, on Oct 30 2009, 06:08 PM, said:

use 0.05% sds and 10-20% methanol in the buffer.

if you have no concern about smaller proteins then you can transfer for a longer period.

but, you will probably never achieve 100% transfer.

Thank you very much for your quick response and for your kindness. Your Information is helpfull for me !!

I did not add in sds yet. I will try to use sds in the buffer.      

By the way, I have  another qusetion.

Can I apply  your conditions for the semi-dry system ?

Would you answer to my question?

[mdfenko]

mTOR, on Nov 2 2009, 03:14 AM, said:

Can I apply  your conditions for the semi-dry system ?

yes.

[mTOR]

Thank you very much!!

I will try it.