I didnt get any proper protocol where i can isolate this Extracelluar protein exclusively, in some methods sonication was used and debris was seperated. protein content was isolated to show correlation between biofilm formation and protein content. the corresponding gene is 9 having kb lenght so overexpression and isolation with tag is tedious. So how sould I proceed ??
plz give me some ideas
Edited by sud373, 29 October 2009 - 09:50 PM.