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#1 happy potter

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Posted 29 October 2009 - 08:24 PM

Hi, everyone, is aminocaproic acid nescessary for doing BN-PAGE?

#2 mdfenko

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Posted 30 October 2009 - 06:30 AM

if it's a component of the buffer then don't omit it and don't try substituting anything else (until you know more about it, if at all).
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#3 lab rat

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Posted 30 October 2009 - 06:59 AM

Keep it in there, as you will need an anion to drive the proteins the correct direction. This is especially important if you are running proteins with a net positive charge.

You can substitute deoxycholic acid (DOC) for the aminocaproic acid.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.




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