Hi, everyone, is aminocaproic acid nescessary for doing BN-PAGE?
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#2
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an elder
Posted 30 October 2009 - 06:30 AM
if it's a component of the buffer then don't omit it and don't try substituting anything else (until you know more about it, if at all).
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
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Why does a science forum not have pictures of mice and rats?
Posted 30 October 2009 - 06:59 AM
Keep it in there, as you will need an anion to drive the proteins the correct direction. This is especially important if you are running proteins with a net positive charge.
You can substitute deoxycholic acid (DOC) for the aminocaproic acid.
You can substitute deoxycholic acid (DOC) for the aminocaproic acid.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia.org/wiki/Python_(programming_language)" target="_blank">http://en.wikipedia.org/wiki/Python_(programming_language)</a>, accessed 25June2009.
