Long real-time Amplicon
Posted 29 October 2009 - 07:18 PM
Posted 30 October 2009 - 04:45 AM
I have experience using the Power Sybr Green 1-step RNA to Ct kit, but the optimal target length is ~50-150 bp and it is unable to handle long targets >a few hundred bps. Does anyone know of a kit or procedure that will allow me to quantitate much longer amplicons (~2 KB)?
You're better off making new primers to amplify a smaller sequence within that 2 kb amplicon.
Posted 30 October 2009 - 06:50 AM