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Inverse /ligation-mediated PCR


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#1 Bing

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Posted 05 July 2002 - 08:03 PM

Can anybody tell me sourses of reference about Inverse PCR or Ligation -mediated PCR method which can be used to amplify 5'-flanking region of a known genomic sequence?

#2 milanoj

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Posted 06 July 2002 - 12:03 PM

Here are a couple.

        PCR Strategy to Obtain the 5' and 3' Flanking Sequences of Partial Clones from lambdaZAP cDNA Libraries
                             
        Li, Q. Nicholas, R.A.
        Published Date: 06/20/1997, Benchmarks, BioTechniques 22:1038-1044

Partial Inverse PCR: A Technique for Cloning Flanking Sequences
                             
        Pang, K. M. Knecht, D.A.
        Published Date: 06/20/1997, Benchmarks, BioTechniques 22:1046-1048





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