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[Bing]

Can anybody tell me sourses of reference about Inverse PCR or Ligation -mediated PCR method which can be used to amplify 5'-flanking region of a known genomic sequence?

[milanoj]

Here are a couple.

        PCR Strategy to Obtain the 5' and 3' Flanking Sequences of Partial Clones from lambdaZAP cDNA Libraries
                             
        Li, Q. Nicholas, R.A.
        Published Date: 06/20/1997, Benchmarks, BioTechniques 22:1038-1044

Partial Inverse PCR: A Technique for Cloning Flanking Sequences
                             
        Pang, K. M. Knecht, D.A.
        Published Date: 06/20/1997, Benchmarks, BioTechniques 22:1046-1048