Hi !
Does anyone there knows how to improve the transfer of long RNAs onto a nylon membrane in northern ? I thought illuminating the gel before transfer with UV light (254 nm), but I would like to know if there is no alternative way to proceed (less agressive for RNAs).
Thanks a lot.
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#2
milanoj
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Posted 05 July 2002 - 09:32 AM
Have you tried alkaline transfer?
#3
John235
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Posted 23 July 2002 - 07:18 PM
Use a trans-blotter (BioRad) and electroblot them
60V for 1 hr. If you are using agarose-formaldehyde gel -- use 0.2X MOPS as the buffer.
