improvment of high MW RNAs in northern blotting
Posted 05 July 2002 - 12:58 AM
Does anyone there knows how to improve the transfer of long RNAs onto a nylon membrane in northern ? I thought illuminating the gel before transfer with UV light (254 nm), but I would like to know if there is no alternative way to proceed (less agressive for RNAs).
Thanks a lot.
Posted 23 July 2002 - 07:18 PM
60V for 1 hr. If you are using agarose-formaldehyde gel -- use 0.2X MOPS as the buffer.