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Secreted protein for loading control


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#1 dzasp

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Posted 29 October 2009 - 06:03 AM

Hi everyone,

I'm researching a secreted protein in culture medium from cell lines of prostate cancer. I run a WB without problems, however I need a secreted protein to normalize the results and loading control (like actin in protein extract).

Are there any protein that always be released to culture medium?

Thank you!
Valentina

Edited by dzasp, 29 October 2009 - 09:39 AM.


#2 bellm

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Posted 03 November 2009 - 09:08 AM

I have read about others using erk (extracellular signal-regulated kinase) as such a loading control.
See Coban 2005, J. Leukocyte Biology 78:647-655. Also see Dittmer 2006, Electrophoresis 27, 2844-2845.

Cheers,
Matt

#3 Feelcontraire

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Posted 18 November 2009 - 06:16 PM

Hi everyone,

I'm researching a secreted protein in culture medium from cell lines of prostate cancer. I run a WB without problems, however I need a secreted protein to normalize the results and loading control (like actin in protein extract).

Are there any protein that always be released to culture medium?

Thank you!
Valentina


Loading controls are fake-prone.

I would recommend you simply performing ponceau stain, scan and lane densitometry.




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