I wish to express siRNAs through vector.
my questions are
Is it possible to add sequences of more than one siRNA in tandem in a vector??
If yes then what should be the desired distance between the two selected siRNA to be diced properly by dicer??
Is their any specific feature of substrate (i.e.dsRNA) required for dicer to recognize n make a staggered cut to give mature siRNA or it acts on dsRNA without any particular recognition pattern..??
Why U6 promoter is always used in siRNA vectors... ??
ur replies will be of kind help as i am new in this field...
Edited by nipun, 29 October 2009 - 12:05 AM.