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How Best to Clean the Sonicator Tip Between Samples?


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#1 Envennom

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Posted 28 October 2009 - 10:13 AM

Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated! :)

Edited by Envennom, 28 October 2009 - 10:13 AM.


#2 lab rat

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Posted 28 October 2009 - 10:31 AM

What about disposable probe covers? They make them for thermometers, but maybe they will work for sonicators.

http://www.fishersci...uctsItemsFlag=Y

http://www.labsafety...160/?CID=9PP001

http://www.allegrome...11124bc743356ff

Edited by lab rat, 28 October 2009 - 10:35 AM.

42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#3 laurequillo

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Posted 28 October 2009 - 12:14 PM

I clean it between samples with the same buffer i am using to sonicate. And after that with ethanol
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#4 Vini

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Posted 28 October 2009 - 10:12 PM

Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated! ;)



i just wipe the tip with 70% EtOH.

#5 Hielscher_Ultrasonics

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Posted 23 November 2009 - 07:56 AM

Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated! :rolleyes:


Envennom,

wiping with ethanol or sonication of ethanol in a beaker is an effective way.

Alternatively, you could sonicate your samples in a sample vial, such as an Eppendorf 1.5mL. Hielscher offers a product called VialTweeter for this. This allows for the sonication of up to eight samples at a time. No wetted probe --> no cross-contamination, no cleaning/rinsing/wiping.

Save time and focus on your research instead of performing tedious cleaning tasks :P

#6 Julie123

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Posted 14 April 2010 - 08:20 PM

Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated! ;)


Envennom,

wiping with ethanol or sonication of ethanol in a beaker is an effective way.

Alternatively, you could sonicate your samples in a sample vial, such as an Eppendorf 1.5mL. Hielscher offers a product called VialTweeter for this. This allows for the sonication of up to eight samples at a time. No wetted probe --> no cross-contamination, no cleaning/rinsing/wiping.

Save time and focus on your research instead of performing tedious cleaning tasks :)

Would cleaning with autoclaved water be sufficient to avoid cross contamination?




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