Edited by Envennom, 28 October 2009 - 10:13 AM.
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5 replies to this topic
#1
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Posted 28 October 2009 - 10:13 AM
Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated!
#2
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Why does a science forum not have pictures of mice and rats?
Posted 28 October 2009 - 10:31 AM
What about disposable probe covers? They make them for thermometers, but maybe they will work for sonicators.
http://www.fishersci.com/wps/portal/ITEMDE...uctsItemsFlag=Y
http://www.labsafety.com/Welch-Allyn-SureT...160/?CID=9PP001
http://www.allegromedical.com/diagnostic-p...11124bc743356ff
http://www.fishersci.com/wps/portal/ITEMDE...uctsItemsFlag=Y
http://www.labsafety.com/Welch-Allyn-SureT...160/?CID=9PP001
http://www.allegromedical.com/diagnostic-p...11124bc743356ff
Edited by lab rat, 28 October 2009 - 10:35 AM.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia.org/wiki/Python_(programming_language)" target="_blank">http://en.wikipedia.org/wiki/Python_(programming_language)</a>, accessed 25June2009.
#3
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The Goddamn Batman!
Posted 28 October 2009 - 12:14 PM
I clean it between samples with the same buffer i am using to sonicate. And after that with ethanol
"He must be very ignorant for he answers every question he is asked" Voltaire
"This is SPARTA!"
"I´m the goddamn batman"
"This is SPARTA!"
"I´m the goddamn batman"
#4
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Posted 28 October 2009 - 10:12 PM
Envennom, on Oct 28 2009, 11:43 PM, said:
Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated!
i just wipe the tip with 70% EtOH.
#5
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Posted 23 November 2009 - 07:56 AM
Envennom, on Oct 28 2009, 11:13 AM, said:
Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated!
Envennom,
wiping with ethanol or sonication of ethanol in a beaker is an effective way.
Alternatively, you could sonicate your samples in a sample vial, such as an Eppendorf 1.5mL. Hielscher offers a product called VialTweeter for this. This allows for the sonication of up to eight samples at a time. No wetted probe --> no cross-contamination, no cleaning/rinsing/wiping.
Save time and focus on your research instead of performing tedious cleaning tasks
#6
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Posted 14 April 2010 - 08:20 PM
Hielscher_Ultrasonics, on Nov 23 2009, 08:56 AM, said:
Envennom, on Oct 28 2009, 11:13 AM, said:
Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated!
Envennom,
wiping with ethanol or sonication of ethanol in a beaker is an effective way.
Alternatively, you could sonicate your samples in a sample vial, such as an Eppendorf 1.5mL. Hielscher offers a product called VialTweeter for this. This allows for the sonication of up to eight samples at a time. No wetted probe --> no cross-contamination, no cleaning/rinsing/wiping.
Save time and focus on your research instead of performing tedious cleaning tasks
Would cleaning with autoclaved water be sufficient to avoid cross contamination?
