I´ve got some problems with my protein. I run an SDS-PAGE gel and I identified my almost pure protein. The following day, in order to follow with the experiment I tried to run a Gel filtration chromatography and I discovered that my protein had disappeared. I measure the concentration with the Nanodrop and there was no protein. Just to test, I run again the protein-loading buffer mixture stored from the last SDS-PAGE and surprinsingly, the protein didn´t appear in the gel. Actually, it was no protein in the gel, it didn´t even appear some shorter fragments. The only step between both electrophoresis was a freezing at -20ºC. We have no mix the tubes names and we are sure of their identity. Could someone give us any advice?
Thank you very much
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